Übersetzung für "Intergenic" in Deutsch

Identification or creation of new unique restriction sites which are located in an intergenic, non-coding part.

By DNA sequencing, it is then possible to determine the sequence of the hypervariable intergenic regions of the microorganism in question.

Commission Implementing Regulation (EU) No 489/2013 of 27 May 2013 amending the Annex to Regulation (EU) No 37/2010 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, as regards the substance double stranded ribonucleic acid homologous to viral ribonucleic acid coding for part of the coat protein and part of the intergenic region of the Israel Acute Paralysis Virus [1] is to be incorporated into the EEA Agreement.

An application for the establishment of MRLs for double stranded ribonucleic acid homologous to viral ribonucleic acid coding for part of the coat protein and part of the intergenic region of the Israel Acute Paralysis Virus for bees has been submitted to the European Medicines Agency.

Table 1 of the Annex to Regulation (EU) No 37/2010 should therefore be amended to include the substance double stranded ribonucleic acid homologous to viral ribonucleic acid coding for part of the coat protein and part of the intergenic region of the Israel Acute Paralysis Virus for bees while establishing the absence of the need to establish a MRL, applicable to honey.

The Committee for Medicinal Products for Veterinary Use has recommended that for this pharmacologically active substance a standard pharmacological and toxicological approach, including the setting of a level of acceptable daily intake, is not appropriate and that there is no need to establish an MRL, applicable to honey, for double stranded ribonucleic acid homologous to viral ribonucleic acid coding for part of the coat protein and part of the intergenic region of the Israel Acute Paralysis Virus for bees.

The primers cg0054 — 9.p and cg0054 — 10.p enable a 1032 bp DNA fragment to be amplified if the wild-type arrangement is present in the intergenic region.

Using a further primer (QlysC_WT_P2) in the same reaction mixture, which is capable of annealing to the lysC gene, enables a 1330 bp DNA fragment to be amplified, whereby integration of the synthetic operon PgapWT_lysCT311I_asd, Pgap3_lysCT311I_asd or Pg3N3_lysCT311I_asd into the intergenic region IBcg0054 is specifically detected.

This involved amplifying from the chromosomal DNA of the colonies a DNA fragment which carries the surrounding region of the intergenic region IBcg0054 and part of the inserted arg gene.

Alternatively, an expression cassette according to the invention can also be integrated in an intergenic region in the chromosome, which has no encoding function, or at another gene site, the other gene site preferably being a nucleotide sequence on the chromosome, which is not essential for the growth of the cells and the production of the amino acid or keto acid.

In this connection it is furthermore preferable for components i) and ii) to be formed by the intergenic region from E. coli, which is located between soxR and soxS and which comprises the SoxR binding sequence, the soxS promoter sequence following the SoxR binding sequence and a sequence following the soxS promoter sequence, which corresponds at the level of the mRNA to a ribosome binding site, or by a nucleic acid sequence homologous to this.

The hybridisation takes place under stringent conditions, that is to say only hybrids in which the probe, for example the nucleotide sequence complementary to soxR or soxS or the intergenic region of soxRS from E. coli, and the target sequence, i.e. the polynucleotides treated with the probe, are at least 70% identical.

With the primer pairs SoxS_for_SphI (SEQ. ID. No. 04) and SoxR_rev_SalI (SEQ. ID. No. 05) and chromosomal DNA from E. coli DIS5a as the template, the gene soxR as amplified together with the intergenic region of soxR-soxS and the first 63 nucleotides of soxS.

Said synthetic operon is integrated via homologous recombination into an intergenic region within the Corynebacterium glutamicum chromosome in such a way that the insertion results in an increase in the copy number of the lysC and asd genes.

In addition to the restriction cleavage sites of the enzymes AvrII and NsiI, in a central position within the intergenic region for the insertion of various DNA sequences, flanking cleavage sites for the restriction endonucleases MunI and HindIII were generated for procedures of subcloning the entire fragment.

The mutation in the upstream region of the gap gene is characterized on the basis of a DNA cassette containing a synthetic operon of the lysC and asd genes under the control of the respective promoters PgapWT, Pgap3 and Pg3N3, which is inserted into the intergenic region described in example 2.

Under the conditions chosen for the PCR reaction, the combination of primers cg0054 — 9.p and cg0054 — 10.p does not result in an amplicon in the case of integration into the intergenic region.

The organism-specific oligonucleotides described in the present invention for the detection of bacteria of the genus Staphylococcus, especially bacteria of the species Staphylococcus aureus, correspond to the 23S/5S intergenic region and the directly adjacent region of the 23S rDNA.

Genome segments which do not encode functional gene products and which do not possess any essential regulatory functions (so-called intergenic segments) are, in principle, suitable for use as insertion sites for foreign DNA.

The genome segment between the gene for the protein kinase and the HD1R gene (sequence listing ID No: 7) may be mentioned as an example of an intergenic region.

If the ITR region, the VEGF gene, the PK gene, the gene which encodes the 10 kDa protein, or the intergenic region between the PK gene and the HD1R gene, is to be used as an insertion site in a PPV, the corresponding regions of the PPV genome, which contain the insertion sites, have to be isolated.