Translation of "Amplifiable" in German

The kit of parts can also contain an amplifiable substance library.

You want to make sure that your used tick-specific DNA is amplifiable?

With increasing MTX concentrations, the dhfr wild type gene can be amplified 1000 fold and more, over many rounds of increasing amplification pressure, while amplifiable dominant markers, such as the mutant dhfr gene or the adenosine deaminase gene, can be amplified only to a limited extent, such as two or three steps.

When optimizing the expression protocol by using the expression plasmids of the invention, the surprising result was obtained that also the hygromycin B phosphotransferase gene is amplifiable.

According to a preferred embodiment of the plasmid of the invention, the sequence for the fusion protein comprises two partial sequences, i.e. a highly amplifiable amplification marker gene, preferably the dihydrofolate reductase gene, and a selection marker gene, preferably the hygromycin B phosphotransferase gene.

This is particularly enabled by the property of a hph amplification potential so that one can speak of a double-dominant selectable and double amplifiable marker protein.

According to a further aspect, the present invention relates to a fusion protein comprised of a highly amplifiable amplification marker and a selection marker.

In a further preferred embodiment, the steps a) to d) are repeated, preferably not more than three times to five times following after each other, whereby from the second round onwards, the binding molecules obtained in step e) are used in step a) instead of the amplifiable library.

In a further preferred embodiment the invention relates to a kit of parts comprising of at least one nonspecific binding competitor as well as a specific binding competitor, especially preferably, furthermore containing an amplifiable substance library.

The separated specific binding molecule, which is either in solution or in suspension, is thus either directly identified by corresponding analysis or is proliferated using an amplification procedure suitable for the chosen amplifiable substance library and subsequently isolated and identified using suitable methods.

A kit of parts, according to the invention, is understood to be a commercially available package containing at least one specific and one nonspecific binding competitor which can also be marketed together with an amplifiable substance library.

For the purposes of the invention, an amplifiable substance library is preferably understood as a library selected from the group consisting of nucleic acid library, phage display-library, antibody library or synthetic-chemical library.

The soluble section of the human ACE2 sequence was cloned into an expression vector into which the amplifiable selection marker DHFR had previously been inserted in order to result in increased expression of the ACE2 gene.

Furthermore the internal control is used to verify that enough amplifiable RNA was extracted during the RNA extraction step.

In a preferred embodiment, the substance library is an amplifiable substance library selected from the group consisting of nucleic acid libraries, phage display-libraries, antibody libraries, peptide libraries and synthetic-chemical libraries, as are well known in this expert field, and which are easy to produce according to the published methods well-known in this field.